N protein alone satisfies the requirement for protein synthesis during RNA replication of vesicular stomatitis virus
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چکیده
منابع مشابه
Inhibition of RNA and protein synthesis in interferon-treated HeLa cells infected with vesicular stomatitis virus.
Synthesis of vesicular stomatitis virus RNA and protein is almost completely inhibited in HeLa cells treated with relatively high doses of human fibroblast interferon. With lower concentrations of interferon virus replication is inhibited, but near normal amounts of virus RNA are found in cells infected at a m.o.i. of 10. All the virus RNA species are found in these cells with the exception of ...
متن کاملStudy of the assembly of vesicular stomatitis virus N protein: role of the P protein.
To derive structural information about the vesicular stomatitis virus (VSV) nucleocapsid (N) protein, the N protein and the VSV phosphoprotein (P protein) were expressed together in Escherichia coli. The N and P proteins formed soluble protein complexes of various molar ratios when coexpressed. The major N/P protein complex was composed of 10 molecules of the N protein, 5 molecules of the P pro...
متن کاملRifampin-susceptible mutant of vesicular stomatitis virus: protein and RNA synthesis.
A rifampin-susceptible strain (VSV Rif+) was selected from the wild vesicular stomatitis virus (VSV) population unsusceptible to rifampin. The VSV Rif+ was blocked in its intracellular replication in the presence of rifampin. In cells, rifampin affected primarily VSV Rif+ transcription, but to a different extent than in a cell-free system. In addition, a decrease in the amount of VSV Rif+ prote...
متن کاملCoupled in vitro transcription and translation of vesicular stomatitis virus messenger RNA ( vesicular stomatitis virus ribonucleoprotein cores / virion - associated RNA polymerase / cell - free protein synthesis / slab gel electrophoresis )
The virion transcriptase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of vesicular stomatitis virus was fully active when ribonucleoprotein cores from purified virions were added to cell-free protein synthesizing systems of eukaryotic origin. Synthesis of mRNA was linear for at least 3 hr and the newly synthesized viral mRNA was efficiently utilized for the synthesis of viral...
متن کاملVesicular Stomatitis Virus G Protein by Swainsonine
Swainsonine, an inhibitor of glycoprotein processing, inhibits the formation of the normal oligosaccharide chain of the G protein of vesicular stomatitis virus. Thus, when vesicular stomatitis virus was grown in baby hamster kidney cells in the presence of swainsonine (15 to 500 ng/ml) and labeled with [2-3H]mannose, the oligosaccharide portion of the G protein was completely susceptible to the...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1984
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.49.2.303-309.1984